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  • 盛菲,纪逸萱,丁海遐,章青,李文.RAC3基因对早期滋养层细胞增殖、迁移和侵袭的影响[J].第二军医大学学报,2018,39(4):399-403    [点击复制]
  • SHENG Fei,JI Yi-xuan,DING Hai-xia,ZHANG Qing,LI Wen.Effect of RAC3 gene on proliferation,migration and invasion of early trophoblast cells[J].Acad J Sec Mil Med Univ,2018,39(4):399-403   [点击复制]
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RAC3基因对早期滋养层细胞增殖、迁移和侵袭的影响
盛菲,纪逸萱,丁海遐,章青,李文*
0
(海军军医大学(第二军医大学)长征医院生殖医学中心, 上海 200003
*通信作者)
摘要:
目的 探讨ras相关C3肉毒杆菌毒素底物3(RAC3)对早期滋养层细胞增殖、迁移和侵袭的影响。方法 收集2015年5月至2016年5月在海军军医大学(第二军医大学)长征医院门诊或住院的不明原因自然流产和人工流产妊娠妇女的绒毛组织各20例,应用qPCR法检测绒毛组织中Rac3 mRNA的表达。取C57/B6小鼠孕6.5、14.5和19.5 d的胎盘组织进行mRNA芯片检测。在早期人滋养层细胞系HTR-8/SVneo中干扰和过表达RAC3后,应用CCK-8法和Transwell实验检测RAC3对HTR-8/SVneo细胞增殖、迁移和侵袭的影响。结果 不明原因自然流产绒毛组织中Rac3 mRNA的表达低于人工流产绒毛组织(P<0.05)。小鼠孕6.5、14.5 d胎盘组织中Rac3 mRNA的表达高于孕19.5 d(P均<0.05)。与对照组相比,干扰RAC3表达后HTR-8/SVneo细胞的增殖、迁移和侵袭能力均减弱(P均<0.05),过表达RAC3后HTR-8/SVneo细胞的增殖、迁移和侵袭能力增强(P均<0.01)。结论 RAC3对早期滋养层细胞的增殖、迁移和侵袭能力具有重要调控作用。
关键词:  自然流产  人工流产  ras相关C3肉毒杆菌毒素底物3  胎盘  滋养层细胞
DOI:10.16781/j.0258-879x.2018.04.0399
投稿时间:2018-03-09修订日期:2018-04-10
基金项目:军队创新重点项目(13CXZ006).
Effect of RAC3 gene on proliferation,migration and invasion of early trophoblast cells
SHENG Fei,JI Yi-xuan,DING Hai-xia,ZHANG Qing,LI Wen*
(Center of Reproductive Medicine, Changzheng Hospital, Navy Medical University(Second Military Medical University), Shanghai 200003, China
*Corresponding author)
Abstract:
Objective To explore the effect of ras-related C3 botulinum toxin substrate 3 (RAC3) on the proliferation, migration and invasion abilities of early trophoblast cells. Methods Villius samples from 20 unexplained spontaneous abortion (SA) and 20 induced abortion (IA) patients were collected between May 2015 and May 2016 in Changzheng Hospital of Navy Medical University (Second Military Medical University). qPCR was used to detect the expression of Rac3 mRNA in the villus tissues. mRNA Chip detection was performed on the placental tissues of 6.5, 14.5 and 19.5 days in mice. After interfering or overexpressing RAC3 in early human trophoblast cell line HTR-8/SVneo, the proliferation, migration and invasion abilities were detected by CCK-8 and Transwell assay, respectively. Results The expression of Rac3 mRNA was significantly lower in the villus tissue of unexplained SA patients than that in the villus tissue of IA patients (P<0.05). Rac3 mRNA expressions were significantly higher in the placental tissues of 6.5 and 14.5 days in mice than that in the placental tissues of 19.5 days (both P<0.05). Compared with the control group, the proliferation, migration and invasion abilities of the HTR-8/SVneo cells were significantly reduced by interfering RAC3 expression (all P<0.05), and the proliferation, migration and invasion abilities were significantly enhanced by overexpressing RAC3 (all P<0.01). Conclusion RAC3 plays an important role in regulating of the proliferation, migration and invasion of early trophoblast cells.
Key words:  spontaneous abortion  artificial abortion  ras-related C3 botulinum toxin substrate 3  placenta  trophoblast cell