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  • 张建花,沈燚,何玉琼,韩婷,秦路平,张巧艳.二仙汤抗骨质疏松有效组分对维甲酸致骨丢失大鼠的影响[J].第二军医大学学报,2018,39(2):165-170    [点击复制]
  • ZHANG Jian-hua,SHEN Yi,HE Yu-qiong,HAN Ting,QIN Lu-ping,ZHANG Qiao-yan.Effect of antiosteoporotic active fraction from Er-Xian Decoction on retinoic acid-induced bone loss in rats[J].Acad J Sec Mil Med Univ,2018,39(2):165-170   [点击复制]
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二仙汤抗骨质疏松有效组分对维甲酸致骨丢失大鼠的影响
张建花1,2,沈燚1,3,何玉琼1,韩婷1,秦路平1*,张巧艳1*
0
(1. 第二军医大学药学院生药学教研室, 上海 200433;
2. 佳木斯大学药学院生药学教研室, 佳木斯 154007;
3. 福建中医药大学药学院, 福州 350122
*通信作者)
摘要:
目的 探讨二仙汤抗骨质疏松有效组分对维甲酸致骨质疏松大鼠的影响。方法 将60只雌性SD大鼠随机分为空白对照组、模型组、戊酸雌二醇(0.2 mg/kg,阳性对照)组和二仙汤抗骨质疏松有效组分低、中、高剂量(50、100、200 mg/kg)组。除空白对照组外,各组大鼠连续14 d灌胃给予维甲酸[70 mg/(kg · d)]诱导骨质疏松症。造模结束后,按分组设计方案对各组大鼠每天灌胃给药(10 mL/kg),连续28 d。用ELISA试剂盒检测大鼠血清和尿液的生物化学指标,显微CT分析股骨远端骨密度和骨小梁微结构的变化。结果 二仙汤抗骨质疏松有效组分可降低维甲酸致骨质疏松大鼠尿液钙的含量,以及血清碱性磷酸酶和抗酒石酸酸性磷酸酶的活性(P<0.05或P<0.01),增加骨密度(高剂量组P<0.05),改善骨小梁微结构的退化。结论 二仙汤抗骨质疏松有效组分可减少维甲酸致骨质疏松大鼠的骨丢失和骨组织微结构的退化。
关键词:  二仙汤  有效成分  骨质疏松  视黄酸  动物模型
DOI:10.16781/j.0258-879x.2018.02.0165
投稿时间:2017-11-10修订日期:2017-12-18
基金项目:国家自然科学基金(81173191),上海市科技支撑计划项目(12401900702).
Effect of antiosteoporotic active fraction from Er-Xian Decoction on retinoic acid-induced bone loss in rats
ZHANG Jian-hua1,2,SHEN Yi1,3,HE Yu-qiong1,HAN Ting1,QIN Lu-ping1*,ZHANG Qiao-yan1*
(1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;
2. Department of Pharmacognosy, School of Pharmacy, Jiamusi University, Jiamusi 154007, Heilongjiang, China;
3. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China
*Corresponding authors)
Abstract:
Objective To explore the effect of antiosteoporotic active fraction from Er-Xian Decoction (EXD) on retinoic acid-induced osteoporosis in rats. Methods Sixty female SD rats were randomly divided into normal control group, model group, estradiol valerate (0.2 mg/kg) group and antiosteoporotic active fraction from EXD low-, middle-and high-dose groups (50, 100, 200 mg/kg). Except for the rats of normal group, the others were intragastrically treated with 70 mg/(kg·d) of retinoic acid for 14 days to induce osteoporosis. Then the rats were treated with estradiol valeratse or antiosteoporotic active fraction from EXD (10 mL/kg) for 28 days. The biochemical parameters in serum and urine of rats were detected by ELISA. The bone mineral density and trabecular bone microstructure of distal femur were analyzed with micro-CT. Results The antiosteoporotic active fraction from EXD significantly decreased calcium content in urine and activities of alkaline phosphatase and tartratse-resistant acid phosphatase in the sera of retinoic acid-induced osteoporotic rats (P<0.05, P<0.01). High-dose of antiosteoporotic active fraction from EXD significantly increased bone mineral density of distal femur (P<0.05). And the antiosteoporotic active fraction from EXD improved the trabecular bone microstructure of distal femur in retinoic acid-induced osteoporotic rats. Conclusion Antiosteoporotic active fraction from EXD can reduce bone loss and deterioration of bone microstructure in retinoic acid-induced osteoporotic rats.
Key words:  Er-Xian Decoction  active ingredient  osteoporosis  retinoic acid  animal model