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  • 刘媛,王文博,邹自英,范泉水,冯子良,熊杰.西南地区部队官兵55型人腺病毒中和抗体的研究[J].第二军医大学学报,2018,39(2):212-215    [点击复制]
  • LIU Yuan,WANG Wen-bo,ZOU Zi-ying,FAN Quan-shui,FENG Zi-liang,XIONG Jie.Study of neutralizing antibody against human adenovirus type 55 in soldiers of Southwest China[J].Acad J Sec Mil Med Univ,2018,39(2):212-215   [点击复制]
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西南地区部队官兵55型人腺病毒中和抗体的研究
刘媛1,王文博2,邹自英1,范泉水2,冯子良2,熊杰1*
0
(1. 成都军区总医院检验科, 成都 610083;
2. 成都军区疾病预防控制中心, 成都 610021
*通信作者)
摘要:
目的 探究西南地区部队官兵55型人腺病毒(HAdV55)抗体流行情况,并检测HAdV55抗体的中和活性,为部队预防和控制HAdV55疫情提供参考依据。方法 收集西南地区部队官兵血清样本325份,用ELISA法检测血清中HAdV55抗体,通过体外中和实验检测抗体阳性血清中和HAdV55感染的活性。结果 西南地区部队官兵体内HAdV55抗体的阳性率较低,325份血清中抗体阳性仅有19份,阳性率为5.85%。19份抗体阳性的血清中,14份在人喉癌上皮细胞系Hep-2具有中和活性,中和效价最高为1:64。结论 西南地区官兵体内HAdV55抗体水平较低,抵抗HAdV55感染的能力较弱。因此一旦出现个别感染者,部队应加强对HAdV55感染的预防和控制,以防止感染的扩散。
关键词:  腺病毒  官兵  中国西南地区  血清  中和抗体
DOI:10.16781/j.0258-879x.2018.02.0212
投稿时间:2017-06-29修订日期:2017-09-07
基金项目:国家自然科学基金(81301445),全军后勤"十二五"重大专项(AWS11L009),全军医学科技青年培育项目(14QNP057),上海市医学生物防护重点实验室开放课题(SKLM1401).
Study of neutralizing antibody against human adenovirus type 55 in soldiers of Southwest China
LIU Yuan1,WANG Wen-bo2,ZOU Zi-ying1,FAN Quan-shui2,FENG Zi-liang2,XIONG Jie1*
(1. Department of Clinical Laboratory, Chengdu Military General Hospital, Chengdu 610083, Sichuan, China;
2. Center of Disease Control & Prevention of Chengdu Military Area Command, Chengdu 610021, Sichuan, China
*Corresponding author)
Abstract:
Objective To investigate the prevalence and neutralizing activity of anti-human adenovirus type 55 (anti-HAdV55) among the soldiers in Southwest China, so as to provide reference for the prevention and control of HAdV55. Methods The sera of 325 soldiers in Southwest China were collected, and the anti-HAdV55 in serum was detected by ELISA. The neutralization activity of antibody positive serum was detected by neutralization test in vitro. Results The positive rate of anti-HAdV55 was low (5.85%, 19/325) among the soldiers in Southwest China. Of the 19 cases of positive serum, 14 cases were able to neutralize HAdV55 infection in human laryngeal epithelial carcinoma cell line (Hep-2 cells) with the highest titer being 1:64. Conclusion The positive rate of anti-HAdV55 is low among the soldiers in Southwest China, which suggests the soldiers in Southwest China have a low resistance to HAdV55 infection. Once the individual infection is found, the troops should strengthen the prevention and control of HAdV55 infection to prevent the spreading of infection.
Key words:  adenovirus  soldiers  Southwest China  serum  neutralizing antibody