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  • 蒋庚西,李春光,赵铁军,李白翎,张冠鑫,陈和忠.沉默Yes相关蛋白1对人非小细胞肺癌细胞增殖和侵袭的影响[J].第二军医大学学报,2017,38(8):1004-1008    [点击复制]
  • JIANG Geng-xi,LI Chun-guang,ZHAO Tie-jun,LI Bai-ling,ZHANG Guan-xin,CHEN He-zhong.Effect of silencing of Yes-associated protein 1 gene on proliferation and invasion of human non-small cell lung cancer cells[J].第二军医大学学报,2017,38(8):1004-1008   [点击复制]
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沉默Yes相关蛋白1对人非小细胞肺癌细胞增殖和侵袭的影响
蒋庚西,李春光,赵铁军,李白翎,张冠鑫,陈和忠*
0
(第二军医大学长海医院胸心外科, 上海 200433
*通信作者)
摘要:
目的 研究Yes相关蛋白1(YAP1)在非小细胞肺癌细胞增殖、细胞周期、迁移、侵袭中的作用。方法 通过慢病毒介导的小干扰RNA靶向抑制人非小细胞肺癌细胞株A549中YAP1基因的表达,采用CCK-8法、流式细胞术分别检测沉默YAP1对A549细胞增殖及凋亡周期的影响,Transwell实验观察沉默YAP1对A549细胞迁移、侵袭能力的影响。结果 沉默YAP1后,A549细胞YAP1 mRNA和蛋白表达均下调(P<0.01),CCK-8实验结果显示沉默YAP1抑制A549的增殖、增加G0/G1期的细胞比例(P<0.01),Transwell实验结果示沉默YAP1抑制A549细胞的迁移、侵袭(P<0.01)。结论 沉默YAP1基因对非小细胞肺癌细胞的恶性生物学特征具有抑制作用,YAP1可能成为肺癌治疗的潜在靶标。
关键词:  肺肿瘤  非小细胞肺癌  Yes相关蛋白1  细胞增殖  细胞侵袭
DOI:10.16781/j.0258-879x.2017.08.1004
投稿时间:2017-04-28最后修改时间:2017-08-08
基金项目:
Effect of silencing of Yes-associated protein 1 gene on proliferation and invasion of human non-small cell lung cancer cells
JIANG Geng-xi,LI Chun-guang,ZHAO Tie-jun,LI Bai-ling,ZHANG Guan-xin,CHEN He-zhong*
(Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author)
Abstract:
Objective To explore the role of Yes-associated protein 1 (YAP1) in the proliferation, cell cycle, migration and invasion of human non-small cell lung cancer (NSCLC) cells. Methods A lenti-siRNA targeting YAP1 (si-YAP1) was used to inhibit the expression of YAP1 gene of human NSCLC cell line A549 cells. CCK-8 assay and flow cytometry were used to determine the effects of silencing of YAP1 expression on A549 cells proliferation and cell cycle, respectively; Transwell assay was used to observe the effect of silencing of YAP1 expression on A549 cell migration and invasion. Results After infection with si-YAP1, the expressions of YAP1 mRNA and protein in A549 cells were significantly down-regulated (P<0.01). YAP1 silencing significantly inhibited A549 cell proliferation, increased the percentage of cells in G0/G1 phase (P<0.01), and significantly decreased the migration and invasion of A549 cells (P<0.01). Conclusion YAP1 silencing can inhibit malignant biological characteristics of NSCLC, which suggests that YAP1 gene may serve as an important target in the gene therapy of lung cancer.
Key words:  lung neoplasms  non-small cell lung cancer  Yes-associated protein 1  cell proliferation  cell invasion