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  • 袁誉铭,孙全,陈学梁,张永红,张景勍.溴吡斯的明磷脂复合物纳米乳体外溶出度与体内吸收的相关性[J].第二军医大学学报,2016,37(5):650-653    [点击复制]
  • YUAN Yu-ming,SUN Quan,CHEN Xue-liang,ZHANG Yong-hong,ZHANG Jing-qing.Pyridostigmine bromide phospholipid complex nanoemulsion: correlation between dissolution in vitro and absorption in vivo[J].Acad J Sec Mil Med Univ,2016,37(5):650-653   [点击复制]
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溴吡斯的明磷脂复合物纳米乳体外溶出度与体内吸收的相关性
袁誉铭1,孙全2,陈学梁1,张永红1,张景勍1*
0
(1. 重庆医科大学重庆高校药物工程研究中心, 重庆 400016;
2. 重庆市中医院药剂科, 重庆 400021
*通信作者)
摘要:
目的 采用Loo-Riegelman法评价溴吡斯的明磷脂复合物纳米乳(PPNE)体内外的相关性。方法 制备PPNE后,采用动态膜透析法研究PPNE体外释放行为,高效液相色谱法(HPLC)测定大鼠口服制剂后的血药浓度,以Loo-Riegelman法计算体内吸收百分数(Fa),并与体外累积释放率作线性回归。结果 PPNE的体外累积释放率(X)与体内Fa(Y)建立的线性方程为Y=1.376 9X-47.543 2,r=0.941 1,r大于相关系数临界值r(6,0.001)(P<0.001)。结论 PPNE体外释放与体内吸收之间具有良好的相关性。
关键词:  溴吡斯的明  纳米乳  体内吸收  体外释放
DOI:10.16781/j.0258-879x.2016.05.0650
投稿时间:2015-08-05修订日期:2016-03-19
基金项目:
Pyridostigmine bromide phospholipid complex nanoemulsion: correlation between dissolution in vitro and absorption in vivo
YUAN Yu-ming1,SUN Quan2,CHEN Xue-liang1,ZHANG Yong-hong1,ZHANG Jing-qing1*
(1. Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, Chongqing 400016, China;
2. Department of Pharmacy, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, China
*Corresponding author)
Abstract:
Objective To investigate the correlation between in vitro release and in vivo absorption of pyridostigmine bromide phospholipid complex nanoemulsion (PPNE) by Loo-Reieglman method. Methods PPNE was prepared and dynamic penetration system was used to analyze its in vitro release. The plasma concentration of pyridostigmine bromide was determined by HPLC after oral administration in rats. Loo-Riegelman method was employed to calculate the in vivo absorption percentage (Fa). Then the regression equation was established between absorption percentage and cumulative release. Results The regression equation was set up as follows: Y=1.376 9X-47.543 2, r=0.9411 (P<0.001). Y represented in vivo absorption percentage and X represented in vitro cumulative release rate. Conclusion There is a significant correlation between in vitro release and in vivo absorption of PPNE.
Key words:  pyridostigmine bromide  nanoemulsion  in vivo absorption  in vitro release