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  • 陆佳,王倩倩,张尉,王蓓蕾,王涛,张林,温小娟,柳国艳,赵杰,肖良,张黎明.不同阳离子对Cyanea capillata水母触手提取物溶血活性的影响(英文)[J].第二军医大学学报,2012,33(3):240-246    [点击复制]
  • LU Jia,WANG Qian-qian,ZHANG Wei,WANG Bei-lei,WANG Tao,ZHANG Lin,WEN Xiao-juan,LIU Guo-yan,ZHAO Jie,XIAO Liang,ZHANG Li-ming.Effect of various cations on hemolytic activity of tentacle-only extract from jellyfish Cyanea capillata[J].Acad J Sec Mil Med Univ,2012,33(3):240-246   [点击复制]
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不同阳离子对Cyanea capillata水母触手提取物溶血活性的影响(英文)
陆佳1△,王倩倩1△,张尉2,王蓓蕾1,王涛1,张林1,温小娟1,柳国艳1,赵杰1,肖良1,张黎明1*
0
(1.第二军医大学海军医学系防化医学教研室,上海 200433
2.第二军医大学海军医学系,上海 200433
共同第一作者
*通信作者)
摘要:
目的 探讨血浆对Cyanea capillata水母触手提取物(TOE)溶血活性的影响和孔道形成在其溶血机制中的作用。方法 在具有相同红细胞浓度的1%稀释的全血和0.5%红细胞悬液中,检测不同浓度(100、200、400 μg/ml)的TOE产生的溶血效应,并在两套体系中分别加入20、50或100 mmol/L的K+、Ca2+、Mg2+、Mn2+、Zn2+、Cu2+、Fe2+、La3+、NH4+等阳离子和离子螯合剂EDTA,观察其对TOE溶血活性的影响。结果 在两套溶血检测体系中,TOE均呈现出剂量依赖性的溶血效应,TOE为200 μg/ml时在红细胞悬液产生的溶血强度高于稀释的全血;Mn2+、Zn2+、La3+、Cu2+、Fe2+和EDTA均明显降低TOE溶血活性(P<0.05),其中以Zn2+的抑制作用最强,而K+、Ca2+、Mg2+、NH4+可不同程度地增强其溶血活性(P<0.05)。结论 血浆对TOE的溶血作用有一定的保护作用,而孔道形成可能在TOE的溶血机制中具有重要作用。
关键词:  水母  霞水母  触手提取物  溶血
DOI:10.3724/SP.J.1008.2012.00240
投稿时间:2012-02-28修订日期:2012-03-05
基金项目:Supported by National Natural Science Foundation of China (41176126, 81000098) and the Natural Science Foundation of Shanghai Municipal Government (10ZR1437900).
Effect of various cations on hemolytic activity of tentacle-only extract from jellyfish Cyanea capillata
LU Jia1△,WANG Qian-qian1△,ZHANG Wei2,WANG Bei-lei1,WANG Tao1,ZHANG Lin1,WEN Xiao-juan1,LIU Guo-yan1,ZHAO Jie1,XIAO Liang1,ZHANG Li-ming1*
(1.Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
2. Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
Co-first authors.
*Corresponding author.)
Abstract:
Objective To investigate the potential role of the pore-formation in the hemolytic activity of tentacle-only extract (TOE) from the jellyfish Cyanea capillata. Methods The effects of various cations, including K+, Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Fe2+ , La3+and NH4+ on the hemolytic activity of TOE were compared in two different test systems: 1% whole blood and 0.45% erythrocyte suspension with the same erythrocyte concentration. Results The hemolytic activities of TOE in both tests were inhibited by Mn2+, Zn2+, La3+, Cu2+ and Fe2+, and were promoted to a minor extent by K+, Ca2+, Mg2+ and NH4+. The chelating agent EDTA also inhibited the hemolytic activity of TOE. Conclusion The pore-formation mechanism might play an important role in the hemolytic activity of TOE.
Key words:  jellyfish  Cyanea capillata  tentacle-only extract  hemolysis