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  • 王茜1,曾抗1*,周浩2,孙乐栋1,周再高1.寻常型天疱疮患者外周血T细胞受体β链可变区CDR3基因克隆谱型分析[J].第二军医大学学报,2009,30(3):291-294    [点击复制]
  • WANG Qian1, ZENG Kang1*, ZHOU Hao2, SUN Le-dong1, ZHOU Zai-gao1.Spectratyping analysis of complementarity-determining region 3 of T-cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with pemphigus vulgaris[J].Acad J Sec Mil Med Univ,2009,30(3):291-294   [点击复制]
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寻常型天疱疮患者外周血T细胞受体β链可变区CDR3基因克隆谱型分析
王茜1,曾抗1*,周浩2,孙乐栋1,周再高1
0
(1.南方医科大学南方医院皮肤科,广州 510515;2.南方医科大学公共卫生学院流行病学系,广州 510515)
摘要:
目的:探讨寻常型天疱疮(PV)患者外周血T细胞受体(TCR)互补决定区(CDR3)基因谱型变化,了解克隆扩增的T细胞与自身免疫性疾病的相互关系。方法:采用免疫扫描谱型分析技术,分析6例正常人外周血单个核细胞TCR β链可变区(BV) CDR3谱型分布特征以及6例PV患者CDR3谱型变化情况。结果:6例正常人TCR BV CDR3谱型均符合正态分布,6例PV患者TCR BV CDR3谱型均出现多态性降低,BV亚家族单/寡克隆增生。结论:PV患者外周血TCR可变区β链CDR3谱型异常可能与PV发病有关。
关键词:  寻常型天疱疮  T淋巴细胞受体  互补决定区3  基因多态性
DOI:10.3724/SP.J.1008.2009.0291
投稿时间:2008-09-11修订日期:2008-10-21
基金项目:国家自然科学基金(30771937).
Spectratyping analysis of complementarity-determining region 3 of T-cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with pemphigus vulgaris
WANG Qian1, ZENG Kang1*, ZHOU Hao2, SUN Le-dong1, ZHOU Zai-gao1
(1. Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515)
Abstract:
Objective:To analyze the changes of the complementarity-determining region 3(CDR3) of T-cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with pemphigus vulgaris (PV), so as to understand the association of proliferated T cells with autoimmune diseases.Methods: Immunoscope spectratyping technique was used to analyze the distribution of TCR β chain CDR3 in 6 normal blood donors and the dominant CDR3 in the PBMCs in 6 PV patients. Results: The spectratypes of TCR BV subfamily CDR3 region was in a Gaussian distribution manner in all the 6 normal blood donors. The 6 PV patients, however, displayed abnormal proliferation, and oligoclonal expansion of the T cells was observed in TCR BV families with different CDR3 sequences. Conclusion: The abnormal spectratypes of TCR BV subfamily CDR3 region in the peripheral blood of PV patients might be related to the pathogenesis of PV.
Key words:  pemphigus vulgaris  T cell receptor  complementarity determining region 3  genetic polymorphism