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  • 翁文浩1,郑军华2,冷俊1,李智1*,李晶华1.5-杂氮-2′-脱氧胞苷对前列腺癌PC3细胞系生长以及抑癌基因GSTP1、RASSF1A转录的影响[J].第二军医大学学报,2009,30(3):256-259    [点击复制]
  • WENG Wen-hao1, ZHENG Jun-hua2, LENG Jun1, LI Zhi1*, LI Jing-hua1.Effects of 5-aza-2′-deoxycitydine on proliferation of prostate cancer cell line PC3 and transcriptional regulation of tumor suppressor gene GSTP1 and RASSF1A[J].第二军医大学学报,2009,30(3):256-259   [点击复制]
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5-杂氮-2′-脱氧胞苷对前列腺癌PC3细胞系生长以及抑癌基因GSTP1、RASSF1A转录的影响
翁文浩1,郑军华2,冷俊1,李智1*,李晶华1
0
(1.同济大学附属第十人民医院检验科,上海 200072;2.同济大学附属第十人民医院泌尿外科,上海 200072)
摘要:
目的:观察DNA去甲基化药物5-杂氮-2′-脱氧胞苷(5-aza-CdR)对前列腺癌细胞株PC3抑癌基因GSTP1和RASSF1A 转录改变以及细胞增殖活性的影响。方法:用不同浓度5-aza-CdR(2、5、10 μmol/L)处理前列腺癌细胞株PC3,利用甲基化特异性PCR(MSP)法检测用药前PC3细胞株GSTP1、RASSF1A启动子甲基化状态;采用实时荧光定量RT-PCR法检测用药过程中GSTP1和RASSF1A mRNA转录改变;用MTT法和流式细胞术检测用药前后PC3肿瘤细胞增殖活性改变。结果: GSTP1、RASSF1A启动子区域出现甲基化现象。与对照组相比,药物组培养24 h后 GSTP1和RASSF1A mRNA表达未发生明显改变;培养48 h后5、10 μmol/L药物组GSTP1和RASSF1A mRNA表达出现上调;72 h后各浓度药物组均检测出两基因mRNA表达升高(P<0.05)。药物组培养24和48 h未出现明显的细胞增殖抑制现象和细胞周期改变;培养72 h后,药物组细胞增殖抑制显著(P<0.05),细胞周期改变明显(P<0.05),阻滞于G0/G1期。结论:GSTP1和RASSF1A基因在PC3前列腺癌细胞系中的失表达可能与其启动子CpG岛高甲基化有关;5-aza-CdR能在一定程度上抑制PC3细胞增殖,干扰细胞周期,提高GSTP1和RASSF1A的转录水平。
关键词:  5-杂氮-2′-脱氧胞苷  GSTP1基因  RASSF1A基因  DNA甲基化  前列腺肿瘤  细胞增殖  细胞凋亡
DOI:10.3724/SP.J.1008.2009.0256
投稿时间:2008-06-23最后修改时间:2008-08-05
基金项目:上海市卫生局科研课题[200646(85)]
Effects of 5-aza-2′-deoxycitydine on proliferation of prostate cancer cell line PC3 and transcriptional regulation of tumor suppressor gene GSTP1 and RASSF1A
WENG Wen-hao1, ZHENG Jun-hua2, LENG Jun1, LI Zhi1*, LI Jing-hua1
(1. Department of Clinical Laboratory, the 10th People’s Hospital, Tongji University, Shanghai 200072, China;2. Department of Urology Surgery, the 10th People’s Hospital, Tongji University, Shanghai 200072)
Abstract:
Objective:To observe the effects of 5-aza-2′-deoxycitydine (5-aza-CdR) on the proliferation and transcription of tumor suppressor gene GSTP1 and RASSF1A in prostate cancer cell line PC3. Methods: The status of 5′CpG island methylation of RASSF1A and GSTP1 genes in PC3 was analyzed by methylation specific PCR (MSP) before treatment with 5-aza-CdR. RASSF1A and GSTP1 mRNA were quantified by real-time PCR during the demethylation process by 5-aza-CdR. MTT assay and flow cytometry were used to examine the proliferative activity of PC3 cells before and after 5-aza-CdR treatment. Results: The 5′CpG island methylation of RASSF1A and GSTP1 genes were detected in human prostate cancer cell line PC3. Compared with control group, RASSF1A and GSTP1 mRNA expression had no significant change 24 h after culture with 5-aza-CdR; their expression was up-regulated 48 h after cultured with 5-aza-CdR, with significant difference found between 5 μmol/L and 10 μmol/L 5-aza-CdR groups. Compared with control group, the expression of RASSF1A and GSTP1 mRNA was significantly increased 72 h after cultured with all concentrations of 5-aza-CdR. MTT assay and cell cycle examination indicated that exposure to 5-aza-CdR for 24 h and 48 h resulted in no obvious growth inhibition and cell cycle change; exposure to 5-aza-CdR for 72 h induced significant growth inhibition (P<0.05) and cell cycle change (P<0.05); and cells were arrested at G0/G1 phase. Conclusion: The 5′CpG island methylation of RASSF1A and GSTP1 genes is probably responsible for RASSF1A and GSTP1 silencing in PC3 cells. 5-aza-CdR can inhibit the proliferation of PC3 cells, disturb the cell cycle, and elevate transcription of GSTP1 and RASSF1A.
Key words:  5-aza-2′-deoxycitydine  GSTP1 gene  RASSF1A gene  DNA methylation  prostatic neoplasms  cell proliferation  apoptosis